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. 2018 Jun 19;11:3521–3531. doi: 10.2147/OTT.S159236

Figure 4.

Figure 4

Curcumin reversed anti-miR-145-induced enhanced effect on the progression of laryngeal squamous cell carcinoma (LSCC) cells.

Notes: (A) TU212 and AMC-HN-8 cells were transfected with anti-miR-145 or anti-miR-con, followed by treatment with different concentrations of curcumin (0, 5, 10, 20 and 40 μM). Cell viability was then detected by MTT assay. TU212 and AMC-HN-8 cells were transfected with anti-miR-145 or anti-miR-con, followed by 20 μM curcumin treatment. (B) miR-145 expression in treated TU212 and AMC-HN-8 cells was analyzed by quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR). (C) Cell viability was detected by MTT assay in treated TU212 and AMC-HN-8 cells. (D and E) Cell migration and invasion abilities were examined by Transwell migration and invasion assays in treated TU212 and AMC-HN-8 cells. (F) Apoptosis of treated TU212 and AMC-HN-8 cells was analyzed by flow cytometry analysis. *. < 0.05.