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. 2018 Jun 20;11:3583–3595. doi: 10.2147/OTT.S160143

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© 2018 Shi et al. This work is published and licensed by Dove Medical Press Limited

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Knockdown of H19 inhibits cell growth, induces apoptosis and affects cell cycle in melanoma cell lines.

Notes: (A) The expression levels of H19 in the normal melanocytes, human epidermal melanocytes (HEM) and melanoma cells lines including CHL-1, UACC904, A-375 and 1205Lu were determined by quantitative real-time PCR (qRT-PCR). (B and C) Knockdown of H19 by siRNA1 and siRNA2 suppressed the expression levels of H19 in A-375 and 1205Lu cells. Cell Counting Kit 8 (CCK-8) assay was performed to determined cell growth, and H19 knockdown significantly inhibited cell growth in (D) A-375 cells and (E) 1205Lu cells. Flow cytometry analysis of cell apoptosis was performed in A-375 cell and 1205Lu cells, and knockdown of H19 induced cell apoptosis in (F) A-375 cells and (G) 1205Lu cells. For the cell cycle analysis, knockdown of H19 decreased cell population in G₀/G₁ phase and increased cell population in S phase as determined in (H) A-375 cells and (I) 1205Lu cells. All the experiments were performed in triplicates. Significant differences compared to Scramble control group were shown as *P<0.05, **P<0.01, ***P<0.001.

Knockdown of H19 inhibits cell growth, induces apoptosis and affects cell cycle in melanoma cell lines.

Notes: (A) The expression levels of H19 in the normal melanocytes, human epidermal melanocytes (HEM) and melanoma cells lines including CHL-1, UACC904, A-375 and 1205Lu were determined by quantitative real-time PCR (qRT-PCR). (B and C) Knockdown of H19 by siRNA1 and siRNA2 suppressed the expression levels of H19 in A-375 and 1205Lu cells. Cell Counting Kit 8 (CCK-8) assay was performed to determined cell growth, and H19 knockdown significantly inhibited cell growth in (D) A-375 cells and (E) 1205Lu cells. Flow cytometry analysis of cell apoptosis was performed in A-375 cell and 1205Lu cells, and knockdown of H19 induced cell apoptosis in (F) A-375 cells and (G) 1205Lu cells. For the cell cycle analysis, knockdown of H19 decreased cell population in G₀/G₁ phase and increased cell population in S phase as determined in (H) A-375 cells and (I) 1205Lu cells. All the experiments were performed in triplicates. Significant differences compared to Scramble control group were shown as *P<0.05, **P<0.01, ***P<0.001.