Figure 4.

Long-term incubation with gabapentinoids does not affect cytoskeletal proteins in PC12 cells.

Notes: Seven-day exposure with gabapentinoids had no effects on morphology or cytoskeletal proteins of PC12 cells (A–F). Cells under control conditions (Co); incubation with 100 µM gabapentin (Gbp); incubation with 100 µM pregabalin (Pgb). Nuclei were stained blue with DAPI. Red staining, α-tubulin; green staining, neurofilament L. (A, C, and E) Phase-contrast images (200× magnification); (B, D, and F) immunocytochemistry images (1,000× magnification).