Fig. 2. Specific probe protein binding to the intended RNA target revealed by an electrophoretic mobility shift assay (EMSA). (A) REX(A)–TERC(Q), (B) REX(A)–G4T(Q), (C) REX(A)–PITX(Q) RNA and their G-quadruplex mutants, REX(A)–TERC(Qm), REX(A)–G4T(Qm), and REX(A)–PITX(Qm) were incubated with either protein GN-REX(A) or RHAU(Q)-GC or both in K+ solution before being resolved by native gel electrophoresis. The –, +, ++, and +++ indicate protein concentrations of 0, 125, 500, and 1250 nM, respectively, for GN-REX(A) and of 0, 250, 1000, and 2500 nM, respectively, for RHAU(Q)-GC. “Qm” in parenthesis indicates the mutated G-quadruplex sequence in this and the rest of the figures.
