Fig. 5. Dependence of BiFC fluorescence enhancement on target RNA expression in E. coli. (A) Time course of the difference in mean fluorescence between cells expressing RNA REX(A)–TERC(Q) and REX(A)–TERC(Qm) in the presence and absence of RNA induction by ATc. (B) RNA level in cells before and after induction with ATc. Data are given in mean ± SD of three experiments. IPTG was added at 0 h with or without ATc. Representative distribution of eGFP fluorescence of corresponding cells is given in Fig. S4B.† .
