Figure 2.

Reversible Covalent Inhibitor. A non-catalytic Cys of RSK2 is targeted by an acrylonitrile and reacts reversibly, presumably with the small fraction of ionized thiolate present at the reaction pH. In this figure and the following figures, the enzyme active site is depicted schematically in green, and for clarity, arrows are shown only for the initial attack leading to covalent bond formation.