Skip to main content
. 2018 May 9;293(25):9854–9868. doi: 10.1074/jbc.RA118.002540

Figure 6.

Figure 6.

Dex increases TGFβ2 in the aqueous humor and in the TM tissues. A and B, total (A) and active (B) forms of TGFβ2 were analyzed by ELISA in aqueous humor from Veh (V)- and Dex (D)-treated mice at 2 weeks. Dex treatment significantly increased both the total and active forms of TGFβ2 in the aqueous humor. Unpaired t test; n = 4 mice. C, anterior segment tissue lysates from Veh- or Dex-treated mice at 3 weeks were subjected to Western blot analysis of TGFβ2. Dex treatment induced the active form of TGFβ2 in the anterior segment tissue lysates (n = 3 in each group). D, immunostaining for TGFβ2 in the anterior segment tissues revealed increased immunostaining for total TGFβ2 in the TM of Dex-treated mice (n = 3, Veh- and Dex-treated mice). Arrows indicate TM tissue. C, cornea; CB, ciliary body. Scale bar = 50 μm. E, Western blot analyses of anterior segment tissues from 5-week-treated mice demonstrate that Dex increased phosphorylation of Smad3. GAPDH was utilized as a loading control.