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. 2018 May 10;293(25):9784–9800. doi: 10.1074/jbc.RA118.003506

Figure 5.

Figure 5.

ABC294640-mediated telomere damage is prevented by the S1P–hTERT complex due to phosphomimicking function of S1P at Ser921 of hTERT. A and B, effects of ectopic expression of WT–, D684A–, S921A–, S921A/D684A–, S921D–, and S921D/D684A–hTERT proteins on telomere damage in the presence/absence of ABC294640 in GM847 cells were detected by immunofluorescence using TIF assay (A). Scale bars represent 100 μm. Images were quantified by ImageJ (B). Data are means ± S.D. from three independent experiments, analyzed by Student's t test (n = 3, *, p = 0.046; **, p = 0.037; ***, p = 0.033). C, effects of ectopically expressed WT–, D684A–, S921A–, S921A/D684A–, S921D–, and S921D/D684A–hTERT proteins on GM847 cell viability in the absence/presence of ABC294640 were measured by trypan blue exclusion assay. Data are means ± S.D. from three independent experiments, analyzed by Student's t test (n = 3, *, p = 0.046; **, p = 0.051; ***, p = 0.044).