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. 2018 May 11;293(25):9674–9684. doi: 10.1074/jbc.RA118.001952

Figure 3.

Figure 3.

LRP1 deficiency inhibits expression of representative LXR-responsive genes and reduces bile acid excretion by liver. Total cellular RNA was isolated from hLrp1+/+ (filled bars) and hLrp1−/− (open bars) mice after feeding the low-fat, low-cholesterol chow or HFHC diet for 16 weeks. The RNA was subjected to RT-qPCR analysis of representative LXR-responsive genes, including Abcg5 (A), Abcg8 (B), and Cyp7a (C). The expression levels were normalized to the levels of cyclophilin in each sample. Cyp7a activity was also assessed by measuring bile acid levels in intestine (D), feces (E), and liver (F) of HFHC-fed mice. The gene expression data represent the mean ± S.E. from 12 mice in each group. Bile acid levels were determined from tissues isolated from seven hLrp1+/+ and six hLrp1−/− mice. * and **, significant differences from hLrp1+/+ mice at p < 0.01 and p < 0.001.