Table 2.
PCR primers
| Primer | Sequence |
|
|---|---|---|
| Forward | Reverse | |
| EMR1 (F4/80) | TGTCTGACAATTGGGATCTGCCCT | ATACGTTCCGAGAGTGTTGTGGCA |
| CD68 | TTTCTCCAGCTGTTCACCTTGA | CCCGAAGTGTCCCTTGTCA |
| CD14 | CAGCCCTCTGTCCCCTCAA | TCTCCATCCCCGCGTTAC |
| MCP1/CCL2 | CTTCCTCCACCACCATGCA | CCAGCCGGCAACTGTGA |
| MIP1α/CCL3 | TTTGAAACCAGCAGCCTTTGCTCC | TCAGGCATTCAGTTCCAGGTCAGT |
| TNFα | ATCCGCGACGTGGAACTG | ACCGCCTGGAGTTCTGGA |
| IL-1β | CTACAGGCTCCGAGATGAACAC | TCCATTGAGGTGGAGAGCTTTC |
| Collagen-1 | CTTCACCTACAGCACCCTTGTG | TGACTGTCTTGCCCCAAGTTC |
| Osteopontin/ENPP1 | TGTTTCGGGTCATACCAGGTAAT | TTAATTCGACTTGCTGTGAATCCT |
| Cyclophilin | TCATGTGGCCAGGGTGGTGAC | CCATTCAGTCTTGGCAGTGC |
| Sonic hedgehog | CAGCGACTTCCTCACCTTCCT | AGCGTCTCGATCACGTAGAAGAC |
| Indian hedgehog | CCACCTTCAGTGATGTGCTTATTT | CGATGACCTGGAAAGCTCTCA |
| Smoothened | GAGGGTGGCCTGACTTTCTG | GAACTTGATGTTTTGTACCTCGTTTG |
| Gli2 | ATGAGAAACCCTACATCTGCAAGAT | GCGTCTGGCCCATGGA |
| Gli3 | ATGGGCACTTATCGGCAAGT | GAATGCGGAGCCTAAGCTTTG |
| α-Actin/ACTA2 | TCCTGACCCTGAAGTATCCGATA | GGTGCCAGATCTTTTCCATGTC |
| Desmin | AACCAGCCCTGAGCAAAGG | CATCCCGGGTCTCAATGG |
| TGFβ-1 | AGAAGTCACCCGCGTGCTA | TGTGTGATGTCTTTGGTTTTGTCA |