Table 1.
Primers used in this study
F means forward; R means reverse.
| Construct | Primers | |
|---|---|---|
| CerS5(N26Q) | F: | GGCTACCCGAGCAAGTGAGCTGGGCTGATC |
| R: | GATCAGCCCAGCTCACTTGCTCGGGTAGCC | |
| CerS5(153–165→ CerS2) | F: | GCATGTGGAGATTCACATTTTATTTAATTGCCTTCATTGCCGGCATGGCCGTCATTGTGGATAAACCTTGGTTCTGGGACATCCGACAG |
| R: | CTGTCGGATGTCCCAGAACCAAGGTTTATCCACAATGACGGCCATGCCGGCAATGAAGGCAATTAAATAAAATGTGAATCTCCACATGC | |
| CerS5(156–165→ CerS2) | F: | GATTCACATTTTATTTATGTATATTCATTGCCGGCATGGCCGTCATTGTGGATAAACCTTGGTTCTGGGACATCCG |
| R: | CGGATGTCCCAGAACCAAGGTTTATCCACAATGACGGCCATGCCGGCAATGAATATACATAAATAAAATGTGAATC | |
| CerS5 (159–165→CerS2) | F: | CATTTTATTTATGTATATTCTGCTATGGAATGGCCGTCATTGTGGATAAACCTTGGTTCTGGGACATCCGACAG |
| R: | CTGTCGGATGTCCCAGAACCAAGGTTTATCCACAATGACGGCCATTCCATAGCAGAATATACATAAATAAAATG | |
| CerS5(299–309→CerS2) | F: | GATTCTGAACACGACCCTCTTTTACCCACTGGAGCTCTATCCTGCCTTCTTTGGCTGGTGGCTCCTCAATGGCC |
| R: | GGCCATTGAGGAGCCACCAGCCAAAGAAGGCAGGATAGAGCTCCAGTGGGTAAAAGAGGGTCGTGTTCAGAATC | |
| CerS2(291–301→CerS5) | F: | CTGGATCCTGCATTGCACCCTGTTTGAGAGTTGGGAGATAATCGGGCCTTATGCTTCATATTACTTCTTCAATTCCATGATGGG |
| R: | CCCATCATGGAATTGAAGAAGTAATATGAAGCATAAGGCCCGATTATCTCCCAACTCTCAAACAGGGTGCAATGCAGGATCCAG | |
| CerS4(291–301→CerS2) | F: | GATCCTCTACACCACATACTACTACCCACTGGAGCTCTATCCTGCCTTCTTTGGCTACTACTTCTTCAACGGGCTTC |
| R: | GAAGCCCGTTGAAGAAGTAGTAGCCAAAGAAGGCAGGATAGAGCTCCAGTGGGTAGTAGTATGTGGTGTAGAGGATC | |
| CerS4(291–301→CerS5) | F: | GATCCTCTACACCACATACTACGAGAGTTGGGAGATAATCGGGCCTTATGCTTCATACTACTTCTTCAACGGGCTTC |
| R: | GAAGCCCGTTGAAGAAGTAGTATGAAGCATAAGGCCCGATTATCTCCCAACTCTCGTAGTATGTGGTGTAGAGGATC |