Figure 2.

cPGES but not mPGES-1 is expressed in macrophages at the injury site. A, LC–MS/MS analysis of PGE₂ in the ipsi- and contralateral sciatic nerves 10 days after the SNI operation. The data are shown as means ± S.E. (n = 6). **, p ≤ 0.01 one-tailed t test. B, representative Western blotting for cPGES and mPGES-1 in ipsilateral and contralateral sciatic nerves 10 days after the SNI operation; bone marrow–derived macrophages stimulated for 24 h with 0.1 μg of LPS served as positive control. C, densitometric analysis of four independent Western blotting experiments as shown in B. The data are shown as means ± S.E. **, p ≤ 0.01, two-tailed t test. D and E, MELC analysis of sciatic nerve 10 days after the SNI operation. The white squares indicate the regions shown for cPGES, IBA1, CD11b, CD45, and F4-80. White arrows indicate cells expressing these five markers. The radar chart (E) displays the percentage of cPGES-expressing cells (100%) that are positive for the respective stated marker (n = 40).