Figure 4.

EP3-deficiency on myeloid cells does not affect mechanical allodynia or immune cell recruitment in the SNI model. A, genotyping of white blood cells from irradiated WT mice having received bone marrow from wild-type (WT/WT) or EP3^−/− (EP3^−/−/WT) mice. The lower PCR band (450 bp) shows WT and the upper and 650-bp EP3 knockouts. B, C57Bl/6N received bone marrow from either WT or EP3-deficient mice before they underwent SNI surgery. Mechanical allodynia was determined before and at the indicated times after surgery. The data are shown as means ± S.E. (WT, n = 7; EP3^−/−, n = 10). C, immune cell numbers determined by FACS analysis at the site of injury at the sciatic nerve in bone marrow–transplanted mice. The data are shown as means ± S.E. (WT, n = 5; EP3^−/−, n = 7).