Table 2.
Antibodies used for MELC and FACS analysis
7-AAD, 7-aminoactinomycin D; MHC, major histocompatibility complex.
| Target/marker | Source | Distributor | Clone |
|---|---|---|---|
| 7-AAD | Calbiochem | ||
| cPGES | Mouse | Cayman Chemical | JJ6 |
| CD4 | Rat | SouthernBiotech | L3T4 |
| CD8a | Rat | BD Pharmingen | 53-6.7 |
| CD11b | Rat | AbD Serotec | M1/70.15 |
| CD11c | Hamster | Miltenyi Biotec | N418 |
| CD25 | Rat | Miltenyi Biotec | 7D4 |
| CD45 | Rat | Miltenyi Biotec | 30F11.1 |
| CD56 (N-Cam) | Rat | R&D Systems | 809220 |
| CD117 | Rat | Miltenyi Biotec | 3C11 |
| F4-80 | Rat | Biolegend | BM8 |
| Fibroblasts | Rat | Santa Cruz | ER-TR7 |
| IB4 | Lectin | Sigma–Aldrich | |
| IBA1 | Mouse | Gene Tex Inc | 1022-5 |
| Ly6C | Rat | Miltenyi Biotec | 1G7.G10 |
| Ly6G | Rat | eBioscience | RB6-8C5 |
| NK1.1 | Mouse | BD Pharmingen | PK136 |
| MHC II | Human | Miltenyi Biotec | REA813 |