Table 1.

Primers used in the study

Shown are sequences of primers used to generate CRT-null lines (KO CRT), the CRT-FS mutants with the KDEL sequence, and the CRT-WT lacking the KDEL sequence (cloning) and used in qPCR analysis.

Primer no.	Primer name	Sequence (5′ to 3′)	Purpose
1	CRTgRNA-1f	GCCTGCCGTCTACTTCA	KO CRT
2	CRTgRNA-2r	TTTGGATTCGATCCAGC	KO CRT
3	CRT-Fwd	CTAGCTAGTTAATTAAGGATCCATGCTGCTATCCGTGCCGC	Cloning
4	CRT-FS + KDELr	CTCGAGGCCTGCAGGAATTCCTACAGCTCGTCCTTGGCCTCAGTCCAGCCC	Cloning
5	CRT-WT-KDELr	CTCGAGGCCTGCAGGAATTCCTAGGCCTGGCCGGGGAC	Cloning
6	CRT-RTf	GAATACAACATCTGGTTTGGTCCCGAC	qPCR
7	CRT-RTr	CAATCAGTGTGTACAGGTGTGTAAAC	qPCR
8	LMP1-RTf	ACAAAACTGGTGGACTC	qPCR
9	LMP1-RTr	GTCTGCCCTCGTTGGA	qPCR
10	β-Actin-RTf	GGCATCGTGATGGACTCCG	qPCR
11	β-Actin-RTr	GCTGGAAGGTGGACAGCGA	qPCR