Figure 4.

HHQ-activated Ca²⁺ responses are blocked by shRNAs directed against T2R14. a–d, mean ± S.E. (error bars) traces of GCaMP6s fluorescence (normalized to initial fluorescence; F/F_o) in H292 cells in the absence (a) or presence (b–d) of co-transfected shRNA plasmids (sequences described under “Materials and methods”). e, bar graph of peak GCaMP6s-measured Ca²⁺ responses from individual experiments during stimulation with HHQ (as shown in a–d), ATP, or PAR-2 agonist 2FLI (134); n = 7–9 (HHQ) or 3–5 (ATP and 2FLI) independent experiments for each condition; **, p < 0.01; n.s., no statistical significance.