Figure 3.

Z_EGFR:03115–IR700DX‐mediated PIT causes cellular death selectively in EGFR+ve cells. Decrease in cell viability as assessed by the CellTiter‐Glo® luminescent cell viability assay 24 or 96 h post‐PIT in 2D cells and 3D spheroids, following 6 h incubation with the Z_EGFR:03115–IR700DX and irradiation with a light dose of 8 or 16 J/cm², was confirmed to be dose dependent and receptor mediated. (a) U87‐MGvIII cells 24 h post‐PIT. (b) MCF7 cells 24 h post‐PIT. (c, d) U87‐MGvIII spheroids 24 and 96 h post‐PIT. (e) WSz4 spheroids 96 h post‐PIT. Data are presented as mean ± SEM (n = 3). Statistical significance in comparison to the control group was determined using an unpaired two‐tailed Student's t‐test with Welch's correction. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 and ****p ≤ 0.0001. [Color figure can be viewed at http://wileyonlinelibrary.com]