Tfh cell responses are increased in the spleens of ME7-infected mice. The mRNA expression levels of genes related to Tfh cell responses in the spleens of control and ME7-infected mice at the indicated times (50, 130, and 200 days after injection). (A) Bcl6, (B) Il21, (C) Il6, (D) Cxcr5, (E) Icos, (F) Pdcd1, (G) Tnfsf13b, (H) Tnfrsf13c, and (I) Cd40. Relative mRNA expression was measured by qRT-PCR and normalized to ActB signals. The data represent the average expression over six separate experiments, using six mice. Error bars represent mean values ± standard deviation. *, p < 0.05, and **, p < 0.01.