Table 3.

Results of one-way MANOVAs with independent factor “Temperature”

Interval	Measure	G10		G15		U28		F30
		Activity	Sleep	Activity	Sleep	Activity	Sleep	Activity	Sleep
EM	F	1.401	3.496	3.796	9.291	10.561	8.074	5.861	9.599
	Df	1/20	1/20	1/24	1/24	1/22	1/22	1/25	1/25
	P	0.250	0.076	0.063	0.006	0.004	0.009	0.023	0.005
LM	F	1.329	2.536	0.579	2.577	4.030	3.295	9.126	9.282
	Df	1/20	1/20	1/24	1/24	1/22	1/22	1/25	1/25
	P	0.263	0.127	0.454	0.121	0.057	0.083	0.006	0.005
MD	F	0.189	1.460	3.390	6.092	0.336	0.051	30.399	49.613
	Df	1/20	1/20	1/24	1/24	1/22	1/22	1/25	1/25
	P	0.668	0.241	0.078	0.021	0.568	0.824	<0.001	<0.001
EE	F	0.130	1.063	2.168	5.043	2.624	0.170	6.719	15.830
	Df	1/20	1/20	1/24	1/24	1/22	1/22	1/25	1/25
	P	0.722	0.315	0.154	0.034	0.119	0.684	0.016	0.001
LM	F	0.735	0.638	1.579	3.758	6.830	1.075	1.713	0.418
	Df	1/20	1/20	1/24	1/24	1/22	1/22	1/25	1/25
	P	0.401	0.434	0.221	0.064	0.016	0.311	0.202	0.524
MN	F	0.112	0.899	37.029	32.116	18.304	20.160	3.561	8.939
	Df	1/20	1/20	1/24	1/24	1/22	1/22	1/25	1/25
	P	0.742	0.354	<0.001	<0.001	<0.001	<0.001	0.071	0.006

Notes: The independent factor “Temperature” (20°C and 29°C). Data on each of four stains in 2 long day experiments (20°C vs 29°C) were analyzed. Activity Measure: Locomotor Activity per 30 min; Sleep Measure: Sleep duration per 30 min. F, Df, P: F-ratio, degree of freedom, and level of significance for the main effects. U28, the strain #28265 maintained in the Bloomington Drosophila Stock Center and collected in Raleigh, NC, USA; F30, the strain from P. Haddrill collected in Montpellier; G10 and G15, two strains from P. Haddrill collected in Ghana.

Abbreviations: DD, constant darkness; LD20:4, light–dark cycle consisting of 20 h of light and 4 h of darkness; EM, early morning (3:00–7:00); LM, late morning (7:00–11:00); MD, middle of the day (11:00–15:00); EE, early evening (15:00–19:00); LE, late evening (19:00–23:00); MN, middle of the night (23:00–3:00).