Figure 3.

miR-410 directly targets TRIM44, and miR-410 is downregulated in osteosarcoma tissues.

Notes: (A) The putative miR-410-binding sequence in the 3′UTR of TRIM44. (B) 293T cells were co-transfected with the WT or MUT luciferase reporter plasmids and miR-410 mimics or negative control. The luciferase activity was evaluated 24 h post-transfection. (C, D) Western blot and qRT-PCR analysis of TRIM44 expression in osteosarcoma cells transfected with miR-410 mimics or negative control. (E) Detection of miR-410 expression in 20 paired osteosarcoma tissues and its corresponding noncancerous tissues. All data are presented as the mean ± SEM of experiments performed in triplicate (**P<0.01).

Abbreviations: WT, wild-type; MUT, mutant; NC, negative control; ns, non-significant.