Figure 4.

miR-410 suppresses cell proliferation, invasion, and EMT of osteosarcoma cells.

Notes: (A) The expression of miR-410 was detected by qRT-PCR in osteosarcoma cells. (B) qRT-PCR analysis of miR-410 expression in MG-63 and U2OS cells after transfection with miR-410 mimics or negative control. Effects of ectopic miR-410 on the proliferation (C), migration (D and E), and invasion (F and G) of MG-63 and U2OS cells were validated by CCK-8, wound healing, and Transwell assays, respectively. (H) Western blotting analysis of EMT markers after transfection with miR-410 overexpression. All experiments were repeated three times and data are shown as mean ± SEM (*P<0.05, **P<0.01).

Abbreviations: EMT, epithelial–mesenchymal transition; CCK8, Cell Counting Kit-8; OD, optical density; NC, negative control.