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Biofilms were grown for 24 h without any disturbance.
Notes: (A) Pseudomonas aeruginosa; (B) Escherichia coli. After 24 h, old culture medium was replaced with different concentrations of C-AgNPs containing fresh medium and incubated for another 24 h. After 24 h of nanoparticles treatment, biofilms were stained with live/dead staining and observed by using fluorescence microscopy.
Abbreviation: C-AgNPs, core–silver nanoparticles.
