Figure 1.

Effects of the H₃ receptor antagonist, E‐162, on neuropathic pain symptoms in male and female mice with CCI. The effects of single i.p. administration of E‐162 (males: 1, 5, 10 and 20 mg·kg⁻¹; females: 10 mg·kg⁻¹) on mechanical (A, von Frey) and thermal (B, cold plate; C, tail flick) stimulus on day 7 following CCI to the sciatic nerve, were evaluated. The data are presented as means ± SEM. The group receiving morphine (M; 10 mg·kg⁻¹, i.p.), was used as a positive control in this study. The analgesic effects of morphine were compared with vehicle (V; water)‐treated mice, which received water for injection as a vehicle. The results of the experiments were statistically evaluated using unpaired sample t‐test (B, D, F), a repeated measures ANOVA (A, C, E; blue symbols) Changes in tested doses at each time point were performed using one‐way ANOVA (A, C, E; green symbols) and were further analysed with Bonferroni's post hoc test. ^* P < 0.05, significantly different from vehicle (25% DMSO/water)‐treated mice; ^# P < 0.05, significantly different from vehicle (water)‐treated mice. The number of animals in each group was as follows: von Frey test [males: E‐162 1, 5, 10 mg·kg⁻¹ (n = 7) and 20 mg·kg⁻¹ (n = 6), V (n = 8) and M 10 mg·kg⁻¹ (n = 7); females: E‐162 10 mg·kg⁻¹ (n = 7) and V (n = 7)]; cold plate test [males: E‐162 1 mg·kg⁻¹ (n = 7) 5, 10 and 20 mg·kg⁻¹ (n = 6), V (n = 8) and M 10 mg·kg⁻¹ (n = 7); females: E‐162 10 mg·kg⁻¹ (n = 6) and V (n = 6)]; and tail flick test [males: E‐162 1, 5, 10 and 20 mg·kg⁻¹ (n = 6), V (n = 8) and M 10 mg·kg⁻¹ (n = 6); females: E‐162 10 mg·kg⁻¹ (n = 7) and V (n = 7)].