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Adenylyl cyclase A and guanylyl cyclase assays. AX2, Gγ/AX2, and GγΔ/AX2 cells were allowed to differentiate for 6 h with cAMP. (A) The adenylyl cyclase activity was recorded after stimulation with 100 nM cAMP stimuli at time 0 s. (B) The activation of adenylyl cyclase by GTPγS was measured. (C) The accumulation of cGMP was recorded after stimulation with 100 nM cAMP. Each assay was repeated at least three times, and their average is presented.
