Figure 7.

YFP-Gβ: GγΔ/AX2 cells fail to carry out chemotaxis. Two representative clones of YFP-Gβ: GγΔ/AX2 were allowed to differentiate for 7 h. (A) YFP-Gβ was predominantly distributed in the cytosol of clone 5 cells. (B) Clone 12 showed wild-type-like distribution of YFP-Gβ. (C) Chemotaxis assay of clone 5 cells was carried out under a Zeiss inverted fluorescent microscope. At time 0 min, a micropipette containing 1 μM cAMP was positioned in a field of cells as indicated by the white star. The photographs were taken at times 0 and 15 min. (D) Chemotaxis assay of clone 12 cells was recorded. At time 0 min, the micropipette was positioned at the white star. These assays were repeated at least three times, and a representative assay is presented.