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. 2018 Apr 14;164(1):339–352. doi: 10.1093/toxsci/kfy090

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© The Author(s) 2018. Published by Oxford University Press on behalf of the Society of Toxicology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contactjournals.permissions@oup.com

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Figure 4. — Cytokinesis-blocked micronucleus formation in HET-1A cells exposed to OH-GQDs. A, Representative images of cells scored using the cytokinesis-blocked micronuleus assay: Normal cell, binucleated cell with micronucleus (MN), binucleated cell with buds (Buds) and binucleated cell with nucleoplasmic bridge (NPB). Scale bar = 4 μm. B, The percentage of nuclear abnormal cells in mitotic cells were counted and represented as mean ± SD from 3 tests. One-way ANOVA was performed. When compared with 0 μg/ml OH-GQDs-treated cells, *** p < .001. C, The percentage of nuclear abnormal cells with MN, NPB, and Buds in mitotic cells were counted separately and represented as mean ± SD. One-way ANOVA was performed. When compared with 0 μg/ml OH-GQDs-treated cells, **p < .01.