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Hydroxyflavonoids as AhR antagonists. Caco2 cells were treated with TCDD and hexahydroxy- (A), pentahydroxy- (B), and tetra-/trihydroxy (C) flavonoids alone or in combination, and inhibition of TCDD-induced CYP1A1 or UGT1A1 gene expression was determined by real-time PCR. Results are expressed as means ± SD for 3 replicate determinations, and significant (p < .05) inhibition of TCDD-induced gene expression is indicated (*).
