Table 4.
TCE Promoted Th1/Th17 Phenotype
| TCE− | TCE− | TCE+ | TCE+ | (1) | (1) | (1) | (2) | (2) | (3) | |
|---|---|---|---|---|---|---|---|---|---|---|
| HFD− | HFD+ | HFD− | HFD+ | vs | vs | vs | vs | vs | vs | |
| (1) | (2) | (3) | (4) | (2) | (3) | (4) | (3) | (4) | (4) | |
| ifng | 33.2 (13.0) | 43.4 (19.8) | 65.8 (34.5) | 78.6 (11.9) | 0.8 | 0.08 | 0.02 | 0.4 | 0.1 | 0.8 |
| Il17 | 21.7 (14.1) | 17.5 (15.4) | 41.8 (26.0) | 111 (77.9) | 0.9 | 0.8 | 0.008 | 0.8 | 0.01 | 0.06 |
| Il4 | 692.6(1441.3) | 329.3 (507.9) | 799.6 (543.2) | 227.2 (189.7) | 0.03 | 0.2 | 0.008 | 0.8 | 0.9 | 0.4 |
| tnfa | 4.1 (1.8) | 26.7 (28.2) | 12.1 (6.1) | 15.4 (7.3) | 0.07 | 0.8 | 0.7 | 0.4 | 0.7 | 0.9 |
Splenic CD4+ T cells were isolated and activated as described in the “Materials and Methods” section. Cells were harvested and processed for RNA and cytokine expression was measured by qRT-PCR. Numbers represent mean and SD fold change values relative to unstimulated CD4+ T cells and normalized by EEF2 housekeeping gene. Data were analyzed by 2-way ANOVA to test for interaction and main effects of the 2 variables (TCE and HFD). Numbers represent mean and SD of the 4 exposure groups (1–4). Data were analyzed by 2-way ANOVA to test for significant interaction of the 2 variables (TCE × HFD). Shown in the table are p values for the 6 pairwise Tukey’s post hoc comparisons numbered by group and conducted as described in the “Materials and Methods” section. Results are statistically significant (p < .05) indicated in bold.