Table 5.

Minimum inhibitory concentrations (MICs) of compound 11a against a broad panel of Gram-positive and Gram-negative bacterial strains.

Compound 11a	MIC (μM)a
Gram-positive bacteria
S. aureus (ATCC 29213)	4.17 μM
S. aureus (MRSA, ATCC 43300)	3.13 μM
E. faecium (VRE, ATCC 70022)	3.13 μM
Gram-negative bacteria
E. coli (ATCC 25922)	No inhibition up to 50 μM
E. coli (MG1655)	No inhibition up to 50 μM
E. coli (BW 25113)	No inhibition up to 50 μM
E. coli (BW 25113) ΔtolCb	No inhibition up to 50 μM
E. coli (BW 25113) ΔacrBb	No inhibition up to 50 μM
E. coli (BW 25113) ΔdapFc	No inhibition up to 50 μM
E. coli (BW 25113) ΔmrcBc	No inhibition up to 50 μM
E. coli (BW 25113) ΔsurAc	No inhibition up to 50 μM
E. coli (ATCC 25922) + 50 μg/mL PAβN	4.6 μM
E. coli (MG1655) + 50 μg/mL PAβN	10.2 μM
E. coli (BW 25113) + 50 μg/mL PAβN	No inhibition up to 20 μM
E. coli (BW 25113) ΔdapFc + 50 μg/mL PAβN	No inhibition up to 20 μM
E. coli (BW 25113) ΔmrcBc + 50 μg/mL PAβN	No inhibition up to 20 μM
E. coli (BW 25113) ΔsurAc + 50 μg/mL PAβN	13.8 μM
E. coli (MG1655) R136-to-C mutant + 50 μg/mL PAβN	No inhibition up to 50 μM

^aMIC (minimum inhibitory concentration that inhibits the growth of bacteria by ≥ 90%) measurements were performed according to the EUCAST guidelines in 3 independent measurements.

^bE. coli strain with mutated efflux pump.

^cE. coli strain with loss-of-function mutation in the gene involved in the cell wall formation.