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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Cold Spring Harb Protoc. 2018 Mar 1;2018(3):pdb.prot097402. doi: 10.1101/pdb.prot097402
CaCl2 0.41 mM
CaNO3 0.3 mM
HEPES-NaOH 15 mM
KCl 1 mM
MgSO4 0.82 mM
NaCl 88 mM
NaHCO3 2.4 mM
Adjust pH to 7.6. Store at room temperature for up to one month