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. 2018 Jun 4;115(25):E5766–E5775. doi: 10.1073/pnas.1804779115

Fig. 1.

Fig. 1.

Effects of stable shRNA-mediated suppression of PHIP in MDA-MB-231 cells. (A) Western analysis of expression of PHIP and other proteins in MDA-MB-231 cells expressing anti-luc shRNA or anti-PHIP shRNA (127738). (B) Colony formation ability of MDA-MB-231 cells expressing anti-luc shRNA or anti-PHIP shRNA (127738) (P < 0.01). (C) Invasion into Matrigel of MDA-MB-231 cells expressing anti-luc shRNA or anti-PHIP shRNA (127738) with corresponding microphotographs (P < 0.001). (D) Quantification of total tumor counts on day 36 in the lungs of nude mice i.v. injected with MDA-MD-231 cells expressing anti-luc shRNA or anti-PHIP shRNA (127738) (P < 0.00001). (E and F) Qualitative immunofluorescence analysis of (E) PHIP and Talin 1 and (F) PHIP and Cyclin D1 in MDA-MB-231 cells expressing anti-luc shRNA or anti-PHIP shRNA (127738). (Scale bar, 20 µm.) (G) Effects of overexpression of TLN1 cDNA or control plasmid on invasive capacity into Matrigel of MDA-MB-231 cells expressing anti-PHIP shRNA (127738) (P = 0.02).