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. 2018 Jun 4;115(25):E5766–E5775. doi: 10.1073/pnas.1804779115

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Fig. 4. — Effects of modulation of PHIP expression in H1703 cells. (A) Western analysis of expression of PHIP and other proteins in H1703 cells overexpressing PHIP cDNA or control plasmid. (B) Effects of overexpression of PHIP cDNA or control plasmid on invasive capacity into Matrigel of H1703 cells (P < 0.05). (C) Effects of overexpression of PHIP cDNA or control plasmid on colony formation ability of H1703 cells (P < 0.05). (D–G) Qualitative immunofluorescence analysis of (D) PHIP and Talin 1, (E) PHIP and Cyclin D1, (F) PCNA and pHH3, and (G) PHIP and Ki67 in H1703 cells overexpressing PHIP cDNA or control plasmid. (Scale bar, 20 µm.)