Fig. 2.

LC differentiation is not impaired due to the lack of GAS6. (A) Epidermal cells were purified from adult Gas6^−/− mice and littermate controls (WT). Representative flow cytometry plots present the frequencies of LCs (langerin⁺) and γδ T cells (γδ TCR⁺) among total CD45⁺ leukocytes. Data are representative of three independent experiments, and each experiment included at least four separately analyzed mice. (B) Whole-mount immunofluorescence staining on epidermal layers prepared from adult Gas6^−/− mice and littermate controls, stained for MHCII (red) and langerin (green). Representative confocal microscopy images are presented, representing one of four independent experiments (n = 3 mice in each experiment). (C) Representative flow cytometry plots demonstrating the expression levels of MHCII, CD86, and langerin on epidermal LC cells of adult Gas6^−/− mice and littermate controls. Data of one of three independent experiments are provided (n = 5 in each experiment). (D and E) Relative expression of various cytokines or TAM receptors and ligands in epidermal layers of adult Gas6^−/− mice and littermate controls were analyzed by RT-qPCR (n = 5 mice). RNA expression data of one of two independent experiments are presented as the mean values ± SD. *P < 0.05.