Fig. 5.
Intact postnatal differentiation of LCs in K5/Pros1fl/fl. Epidermal cells were prepared from 9-d-old (P9) K5/Pros1fl/fl mice and littermate Cre− controls. (A) Representative flow cytometry plots and graph present the frequencies of CD45+MHCII+ cells in the epidermis. (B) Frequencies and absolute numbers (per a half ear) of LCs (CD45+MHCII+langerin+) in the epidermis. Representative flow cytometry histogram demonstrates langerin expression in P9 K5/Pros1fl/fl and Cre− mice, as well as adult Cre− mice. Data are representative of two independent experiments, and each experiment included at least three separately analyzed mice. (C–E) Relative expression of Pros1 and various genes in epidermal layers of (C and D) P9 K5/Pros1fl/fl and Cre− mice, or (E) adult vs. P9 Cre− mice were analyzed by RT-qPCR (n = 5 mice). RNA expression data of one of two independent experiments are presented as the mean values ± SD. Ctrl, control. *P < 0.05.