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. 2018 Jun 4;115(25):6446–6451. doi: 10.1073/pnas.1803220115

Fig. 4.

Fig. 4.

Binding of BCAAs occurs with CTD present in other RSHs. (A, Left) The molecular phylogenetics of 13 RSHs were analyzed using the Maximum Likelihood method in MEGA7. The 13 RSHs were categorized into four groups: bifunctional SpoT from Gammaprotobacteria (pink), bifunctional Rel from Alphaprotobacteria (yellow), monofunctional synthetase RelA from Gammaprotobacteria (green), and bifunctional Rel in Gram-positive species (blue). (A, Right) “+” indicates binding revealed in ITC experiments. (B–G) CTDs of various RSHs titrated with BCAAs using ITC. (B) 20 μM E. coli RelA CTD with 1.5 mM Val. (C) 40 μM R. centenum Rel CTD with 15 mM Val. (D) 40 μM R. centenum Rel CTD with 50 mM Ile. (E) 40 μM B. subtilis Rel CTD with 25 mM Leu. (F) 108 μM S. aureus RelA CTD with 25 mM Leu. (G) 62 μM D. radiodurans RelA CTD with 25 mM Leu.