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. 2018 Jun 5;115(25):6464–6469. doi: 10.1073/pnas.1718003115

Fig. 5.

Fig. 5.

MrsI mediates an anticipatory iron-sparing response in M. tuberculosis. (A) Heat map of genes putatively directly regulated by MrsI in MrsI-inducing stress conditions. (B) Growth curves of M. tuberculosis mrsI knockdown strain with and without CRIPSRi induction in rich medium (Top) and during iron starvation (Bottom; *P < 0.05, unpaired t test). Error bars represent SD of three replicates. (C) Levels of the MrsI target bfrA with and without preexposure to oxidative stress in M. tuberculosis. Three biological replicates of the MrsI knockdown strain were grown with or without oxidative stress for 4 h before iron starvation for 24 h (gray region). Levels of bfrA were measured by using Nanostring. Samples with and without MrsI knockdown (Bottom and Top, respectively) are shown (*P < 0.05 and **P < 0.005, unpaired t test). Error bars represent the SD of three biological replicates.