Skip to main content
. 2018 Jun 4;115(25):E5796–E5804. doi: 10.1073/pnas.1707702115

Fig. 4.

Fig. 4.

ATP-puffing-induced constriction is mediated by purinergic type 2 receptors. (A) After topical application of 0.5 mM PPADS, the puffing pipette is placed in proximity of a near-arteriole site. Red, blue, green, and yellow ROIs are placed at the p.a. and first-, second-, and third-order capillaries to measure the diameter, respectively. (Scale bar: 10 µm.) (B) Time course of the diameter change in each ROI indicated in A. Preconditioning with 0.5 mM PPADS and puffing with 1 mM ATP induces dilation but profoundly attenuates constriction. (C) The amplitude of dilation is not significantly different with and without the application of PPADS among all orders of capillaries. n.s., not significant; P > 0.05, unpaired t test. (D) The amplitude of constriction is significantly different with and without preconditioning with PPADS on the first- and second-order capillaries. *P < 0.05, unpaired t test. All error bars represent SEM.