Fig. 5.

Increased TSLP is produced by keratinocytes in ΔNC16A mice and BP patients. (A) Compared with WT, ear skin from skinΔNC16A mice showed a higher expression of TSLP mainly in epidermis. (B) WT and ΔNC16A keratinocytes were stimulated with mouse TNFα for 24 h. ΔNC16A keratinocytes released significantly higher amount of TSLP in culture medium than WT keratinocytes (n = 6 per group). (C–E) The serum, blister fluids, and lesional skin from BP patients exhibited an increased level of TSLP compared with normal control, blister fluids from herpes zoster, and normal control, respectively (n = 12 per group). (F) The increased expression of TSLP in BP patients is mainly seen in epidermis (n = 9 per group). (Original magnifications: A, 200×; B, 400×.) (Scale bars: A, 100 μm; F, 50 μm.) *P < 0.05. Student’s t test, graph B shows mean ± SE.