Fig. 1.

p53 controls the cell-autonomous functions in CAFs. (A) hTERT-immortalized NFs and CAFs from patient 4731, stably expressing either p53 shRNA or control LacZ shRNA, were subjected to a collagen gel contraction assay in triplicate and imaged after 24 h. (B) Quantification of the collagen gel area, analyzed as in A. Values represent averages ± SEMs from three independent experiments. (C) Cells as in A were grown in trans-well inserts. The lower chamber was loaded with medium containing EGF (10 ng/mL). Sixteen hours later, cells that had migrated across the membrane were stained with crystal violet and photographed. (Scale bars, 500 μm.) (D) Average migration ± SEM from three independent experiments performed as in C; quantification is described in SI Appendix. **P < 0.01.