Fig. 4.

p53 regulates the CAF secretome. (A) Conditioned media of immortalized fibroblasts (patient 4731) were subjected to LC-MS analysis. The heatmap was generated using differentially secreted proteins between CAFshLacZ and CAFshp53 (fold-change >1.5 and adjusted P value <0.05 in four repeats; total = 58 proteins). Colors indicate relative expression (Top bar). (B) Pathway enrichment analysis (GeneAnalytics) for secreted proteins expressed differentially between CAFshLacZ and shp53. (C) Relative abundance of the indicated proteins in CM from immortalized CAFs and NFs, with and without p53 silencing, deduced from the LC-MS analysis. Bars represent normalized LFQ intensities. Values (mean ± SEM) were derived from four independent experiments. *P ≤ 0.05, **P < 0.01, ***P ≤ 0.001 using one-way ANOVA and Tukey post hoc test. LC-MS, liquid chromatography-MS; LFQ, label-free quantification.