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. 2018 Apr 5;24:14. doi: 10.1186/s10020-018-0016-7

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Experimental strategy. a Isolation of circulating DNA (maternal and fetal) from plasma obtained from pregant women at different gestational ages and analysis of fetal sex by qPCR and ddPCR. b An automated system blends oil samples into a cartridge and generates droplets of nanolitre size by a microfluidic system and vacuum generation. c The emulsion is transferred to the thermocycler for the amplification. The presence of the target template in the droplets will be detected by the fluorescence development. d Detection of fluorescence using the Droplet Reader. The system, after the injection of a reader oil, separates and aligns each droplet, which is analyzed by two lasers to detect fluorescence. e Analysis of the data represented in graphs as the fluorescence (FAM or HEX) relative to each droplet (Event Number)