Table 3.
Effect of insulin (100 μU/ml) on cardiac metabolism in Dahl salt-sensitive rats fed a high salt diet (HSD)
| Control | 3 weeks | 6 weeks | 9 weeks | |
|---|---|---|---|---|
| Glycolysis | 1231.3 ±234.8 | 2390.9 ±390.4 | 2090.6 ±647.2 | 2982.2 ±734.1 |
| Glucose Oxidation | 312.2 ±20.6 | 519.0 ±119.7 | 464.8 ±113.6 | 493.0 ±166.7 |
| Palmitate Oxidation | 983.5 ±47.5 | 931.0 ±38.2 | 796.9 ±43.8 | 616.3 ±56.9* |
| Lactate Oxidation | 377.0 ±46.2 | 426.5 ±95.0 | 339.6 ±62.4 | 275.0 ±113.7 |
| Proton Production | 1838.3 ±454.1 | 3743.8 ±558.6 | 3251.6 ±1073.8 | 4978.3 ±1197.6 |
| ATP Production | ||||
| Glycolysis | 2.5 ±0.5 | 4.8 ±0.8 | 4.2 ±1.3 | 6.0 ±1.5 |
| Glucose Oxidation | 9.1 ±0.6 | 15.1 ±3.5 | 13.5 ±3.3 | 14.3 ±4.8 |
| Palmitate Oxidation | 102.3 ±4.9 | 96.8 ±4.0 | 82.9 ±4.6 | 64.1 ±5.9*# |
| Lactate Oxidation | 5.5 ±0.7 | 6.2 ±1.4 | 4.9 ±0.9 | 4.0 ±1.6 |
| Total | 115.6 ±3.3 | 122.8 ±6.2 | 106.4 ±10.2 | 88.3 ±11.0 |
Energy metabolic rates (nmol●g dry wt−1●min−1) were measured during the working heart perfusion. Contribution to ATP production (μmol●g dry wt− 1●min− 1) was calculated from the metabolic rates assessed via the isolated working heart perfusion in Dahl salt-sensitive rats. These results are from Dahl salt-sensitive rats fed a low salt diet, 0.3% NaCl (Control), or a high salt diet, 8% NaCl, for 3, 6, or 9 weeks. n = 3–5 * p < 0.05 compared to Control. # p < 0.05 compared to 3 weeks. Values shown as mean ±SEM