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. 2001 Oct;12(10):3226–3241. doi: 10.1091/mbc.12.10.3226

Figure 5.

Figure 5

NPL4 function is required for OLE1 transcription. Northern analysis was performed on total RNA isolated from ole1Δ (lane 1), wild-type (WT; lanes 2–4), npl4-1 (lanes 5–7), npl4-2 (lanes 8–10), cdc48-3 (lanes 11–13), ufd1-1 (lanes 14-16), and rpn4Δ cells (lanes 17–19) with OLE1- and ACT1-specific DNA probes. Cells were grown in YEPD media at 25°C with or without supplemented UFAs. For wild-type cells, the temperature-sensitive-strains npl4-1, npl4-2, and cdc48-3, and non–temperature sensitive strains ufd1-1 and rpn4Δ, samples were also collected after shifts to 37°C for 60 min. A normalized OLE1/ACT1 ratio was calculated (see MATERIALS AND METHODS) for each sample to allow for quantitation of the relative changes in OLE1 expression among samples.