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. 2018 Jun 25;13(6):e0199711. doi: 10.1371/journal.pone.0199711

Fig 5. Measurement of cytokine production in heart tissue and serum in response to I/R injury.

Fig 5

Following I/R injury, heart tissue and blood were harvested from WT and CARD9-/- sham controls, WT+I/R and CARD9-/-+I/R mice. TNF-α and IL-6 were measured using commercial ELISA kits. A, Concentrations of TNF-α and IL-6 in heart tissue; B, concentrations of TNF-α and IL-6 in serum. I/R injury significantly increased the production of TNF-α and IL-6 compared to sham controls. CARD9 knockout significantly attenuated the levels of these two cytokines in heart tissue and serum following I/R injury. Mean ± SEM, n = 4/group, ***p < 0.001 vs. sham controls; ##p < 0.01, ###p < 0.001 vs. WT.