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. 2018 Jun 25;13(6):e0199632. doi: 10.1371/journal.pone.0199632

Fig 4. The effect of Matrigel thickness on gene markers of differentiation and syncytial fusion.

Fig 4

Normalized mRNA levels of (A) GCM1, (B) PL, (C) ERVWE1, (D) ERVFRD1, (E) CGA, and (F) CGB after 7 days of growth on various surface thicknesses. (E) Normalized protein levels of secreted hCGβ in media. Significant differences between treatment groups determined by one-way ANOVA followed by Tukey’s post-test; n≥3. Significant differences between means determined by post-tests were indicated by * (p<0.05), ** (p<0.01), or *** (p<0.001).