Fig 5. Recombination events in biofilm co-culture.

Circos plot visualizes the whole S. pneumoniae CP2215 genome with bp coordinates in 50-kb intervals labelled outside of the ring. Red lines in the first inner ring represent SNPs between strain CP2215 (donor strain) and R36KAN (recipient strain for biofilm experiments). Subsequent inner rings represent recombinants from the biofilm experiments. Lines on the inner rings represent SNPs transferred from donor to the recombinant. As expected, each recombinant strain carries one recombination event for each selected marker (Nov^R near 770 kbp and/or Spc^R near 212 kbp), plus some additional events nearby due to linkage, plus some events much farther away due to congression. Positions marked as S, N indicate positions of the Nov^R and Spc^R selective markers.