Figure 1.
Aloin does not affect cell growth in murine macrophages: (A) Chemical structure of aloin; (B) RAW 264.7 cells were treated with various concentrations of aloin (100 μM, 200 μM, 300 μM, 400 μM, and 500 μM) for 24 h. The total number of viable cells was determined by methyl thiazolyl tetrazolium (MTT) assay. Values are mean ± SD of three independent experiments; (C) RAW 264.7 cells were treated with various concentrations of aloin and lipopolysaccharide (LPS) (100 ng/mL) for 24 h. The total number of viable cells was calculated. Values ARE mean ± SD of three independent experiments.