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. 2018 Feb 26;23(3):517. doi: 10.3390/molecules23030517

Figure 2.

Figure 2

Aloin inhibits the LPS-induced expression of IL-6 and TNF-α RAW 264.7 cells were pre-treated with aloin for 2 h prior to LPS (100 ng/mL) stimulation. Cell-free supernatants were collected to detect (A) Interleukin 6 (IL-6) and (B) Tumor necrosis factor alpha (TNF-α) concentrations via ELISA after LPS treatment for 9 h and 24 h, respectively. The mRNA expression levels of (C) IL-6 and (D) TNF-α were determined by qPCR in RAW264.7 cells pre-treated with aloin (400 μM) followed by LPS (100 ng/mL) stimulation for 6 h. The data shown are the means ± SD of three experiments. ### p < 0.001 is significantly different from the control. * p < 0.05, ** p < 0.01, and *** p < 0.001 are different from the LPS alone.