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. 2018 Feb 26;23(3):517. doi: 10.3390/molecules23030517

Figure 3.

Figure 3

Aloin inhibits nitric oxide (NO) production and the expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). (A) RAW 264.7 cells were pre-treated with aloin for 2 h prior to LPS (100 ng/mL) treatment. Cell-free supernatants were collected to determine NO production using the Griess reaction after LPS treatment for 24 h. The mRNA levels of (B) iNOS and (C) COX2 were determined by qPCR; (D) RAW 264.7 cells were pre-treated with or without 400 μM aloin for 2 h, and then stimulated with LPS (100 ng/mL) for 12 h, 18 h and 24 h. Levels of iNOS and COX-2 proteins were determined by Western blotting. The results shown are the means ± SD of three experiments. #p < 0.05 and ### p < 0.001 are significantly different from the control. * p < 0.05, ** p < 0.01, and *** p < 0.001 are different from the LPS alone.