Skip to main content
. 2018 Feb 26;23(3):517. doi: 10.3390/molecules23030517

Figure 4.

Figure 4

Aloin inhibits LPS-induced NF-κB p65 phosphorylation and acetylation. (A) RAW 264.7 cells were pre-treated with various concentrations of aloin for 2 h, and stimulated with LPS (100 ng/mL) for 2 h. The protein expression levels of phospho-p65, total p65, MyD88, TLR4, TLR7, phosphor-IκBα, phospho-IKKα/β, phospho-Msk1, phospho-p38 and total p38 was determined by Western blotting; (B) Cells were pre-treated with or without aloin (400 μM) for 2 h, and stimulated with LPS (100 ng/mL) for 15 min, 30 min, 60 min, or 120 min. The protein levels of phospho-p65 and total p65 were analyzed by Western blotting; (C) Immunoblotting analysis of acetylated p65, total p65, and HDAC1 in RAW 264.7 cells treated with aloin and LPS at indicated time points.